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Warrington, PA; Eppelheim, Germany, March 23, 2005 - Jobwerx News - A common method for separating proteins and nucleic acids is by polyacrylamide gel electrophoresis (PAGE). Polysciences produces an ultra acylamide monomer by a proprietary method for use in preparing homogeneous and reproductible electrophoretic gels.

The preparation of polyacrylamide gels is by a free radical dependent copolymerization of acrylamide monomer with a crosslinker. A common crosslinker. A common crosslinker in PAGE is bisacrylamide (bis). Also required are an initiator, commonly ammonium persulfate to supply free radicals, and N,N,N',N'-Tetramethylethylenediamine (TEMED) as the catalyst. The ration of acrylamide to corsslinker determines the pore size of the gel. This is turn determines the size range of molecules that can be separated in that gel.

This proprietary method from Polysciences provides purification results in a product more highly purified than that proceed in recrystalization processes. Polysciences' Chemzymes Ultra Pure(R) Acrylamide is available as the monomer or as premixed powder and solutions in the most popular acrylamide:bis ratios for use in PAGE.

High purity, low conductive, highly soluble acrylamide will produce high quality, reproducible polyacrylamide gels. Acrylamide with low levels of acrylic acid and contaminants is required to prevent unwanted chain termination during polymerization. High levels of acrylic acid can coploymerize with linear polyacrylamide during gel formulation to cause local areas of pH changes in the resultant gels. This can cause streaking and smearing of bands during migration through the gel and can be especially problematic in fluorescent DNA sequencing gels. Both acrylamide and bis, especially in water, can breakdown into acrylic acid over time, affecting the mobility of molecules through the gel. To minimize acrylic acid formation, prepare acrylamide and acrylamide:bis solutions in quantities for use within a month and keep solutions protected from light.

See Polysciences, Inc. at IMAPS 2005 in Philadelphia, PA, September 25-29, 2005


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